Information of DNA-Dye Methylene Blue

Product name:DNA-Dye Methylene Blue
CAS:  61-73-4
Molecular Formula: C16H18ClN3S
Molecular weight:319.85 g/mol
Description of DNA-Dye Methylene Blue:
Synonym:Methylene blue DNA staining reagent
Protocol for staining with DNA-Dye Methylene blue:
Approx. 50 mI 1X DNA-Dye Methylene blue staining solution are required to stain an agarose gel with the dimensions of approx. 80 x 60 mm (width x length) and a thickness of 3 - 4 mm. The gel should be trimmed down appropriately, with the edge of a ruler for example, to remove areas of the gel that were not used for DNA separation. The staining is performed in an adequately sized dish, in which the gel is floated to a depth of about 5 mm with dye solution. To prepare the DNA-Dye Methylene blue staining solution for use, 250 μI are dissolved in 50 mI water. The gel is left to stain for about 20 minutes and the dish is shaken occasionally. To reveal the bands (different staining intensities) the gel is destained with tap water several times for about 5 - 10 minutes. The blue-stained DNA bands are clearly visible in transmitted light and can be measured with a ruler or documented photographically. Methylene blue does not stain DNA permanently: depending on the thickness of the gel and the storage temperature, the DNA bands (especially smaller fragments) may lose their staining after some time.
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